CD117 Antibody

CD117 is a cytokine receptor expressed on the surface of hematopoietic stem cells as well as other cell types. Altered forms of this receptor may be associated with some types of cancer. It is a receptor tyrosine kinase type III, which binds to stem cell factor (a substance that causes certain types of cells to grow), also known as steel factor or c-kit ligand. When this receptor binds to stem cell factor (SCF) it forms a dimer that activates its intrinsic tyrosine kinase activity, that in turn phosphorylates and activates signal transduction molecules that propagate the signal in the cell. Signalling through CD117 plays a role in cell survival, proliferation, and differentiation. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow Cytometry: 1:10-1:50

UniProt

P10721

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 680-711 from the human protein was used as the immunogen for this CD117 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the CD117 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD117 antibody is available for research use only.

Storage Conditions

Aliquot the CD117 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the CD117 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Pig

Image Legend

CD117 antibody flow cytometric analysis of 293 cells (green) compared to a negative control cell (blue) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.