PGK1 Antibody

Also known as ATP:3-phosphoglycerate 1-phosphotransferase, this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. Phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and is proposed to participate in the angiogenic process as a disulfide reductase. Mutations in PGK1 may be associated with hemolytic anemia.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50

UniProt

P00558

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 305-334 from the human protein was used as the immunogen for this PGK1 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC, WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PGK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PGK1 antibody is available for research use only.

Storage Conditions

Aliquot the PGK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the PGK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Primate

Location

Nuclear, cytoplasmic

Image Legend

Western blot analysis of lysate from A431, HeLa, U-937, BA/F3 cell lines using PGK1 diluted at 1:1000 for each lane. Expected/observed molecular weight ~44kDa.