PKM2 Antibody

PKM2 is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:200, Western blot: 1:1000, IHC (Paraffin) : 1:50-1:1:100

UniProt

P14618

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A portion of amino acids 476-505 from the human protein was used as the immunogen for this PKM2 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the PKM2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PKM2 antibody is available for research use only.

Storage Conditions

Aliquot the PKM2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the PKM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of lysate from 1) human HeLa, 2) human A549, 3) mouse NIH3T3, 4) mouse C2C12, and 5) rat PC-12 lysate using PKM2 antibody at 1:1000. Predicted molecular weight ~58 KDa.