Pyruvate Kinase Antibody

PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:2000

UniProt

P14618

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 121-151 from the human protein was used as the immunogen for this Pyruvate Kinase antibody.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the Pyruvate Kinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Pyruvate Kinase antibody is available for research use only.

Storage Conditions

Aliquot the Pyruvate Kinase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Pyruvate Kinase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Rat, Rabbit

Image Legend

Western blot testing of Pyruvate Kinase antibody at 1:2000 dilution. Lane 1: MCF-7 lysate; 2: HeLa; 3: NIH3T3; 4: PC-12; Predicted molecular weight ~ 58 kDa.