SYVN1 Antibody (HRD1)

HRD1/SYVN1 is a ubiquitin ligase whose expression is induced by the unfolded protein response (UPR) following endoplasmic reticulum stress. Expression of SYVN1 protects cells from apoptosis by inducing degradation of abnormally processed proteins that accumulate in the endoplasmic reticulum. It is expressed in many tissues, strongly expressed in brain, pancreas, liver, kidney and skeletal muscle. Amano T, et al. reported that Synoviolin/Hrd1 (expressed in rheumatoid synovium) is a novel causative factor for arthropathy by triggering synovial cell outgrowth through its antiapoptotic effects.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:200-1000, IHC (Paraffin) : 1:50-1:100

UniProt

Q86TM6

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 586-617 from the human protein was used as the immunogen for this SYVN1 antibody.

Clonality

Polyclonal

Isotype

Applications

IF, IHC, WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the SYVN1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SYVN1 antibody is available for research use only.

Storage Conditions

Aliquot the SYVN1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the SYVN1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Fluorescent confocal image of HeLa cells stained with SYVN1 antibody. Primary Ab (1:200, 2 h at room temperature) ; Alexa Fluor 488 conjugated donkey anti-rabbit Ab (green) was used as secondary (1:1000, 1h) . Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min) .