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EIF4B Antibody

EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. It also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50

UniProt

P23588

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 400-429 from the human protein was used as the immunogen for this EIF4B antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the EIF4B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This EIF4B antibody is available for research use only.

Storage Conditions

Aliquot the EIF4B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the EIF4B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of EIF4B antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4B gene (2) .

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