NUP50 Antibody

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. NUP50 may serve as a binding site on the nuclear side of the pore complex for export receptor-cargo complexes. It interacts with multiple transport receptor proteins including p27Kip1. This interaction is required for correct intracellular transport and degradation of p27Kip1.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

Q9UKX7

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 432-461 from the human protein was used as the immunogen for this NUP50 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the NUP50 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This NUP50 antibody is available for research use only.

Storage Conditions

Aliquot the NUP50 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the NUP50 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Rat

Image Legend

Western blot analysis of NUP50 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUP50 gene (2) .