Bax Antibody
Bax belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bax forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of the Bax gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50
UniProt
Q07812
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 41-76 (BH3 domain) from the human protein was used as the immunogen for this Bax antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the Bax antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Bax antibody is available for research use only.
Storage Conditions
Aliquot the Bax antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the Bax antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine
Image Legend
Bax antibody western blot analysis in HL-60 lysate
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