AMT-NHS

AMT-NHS is an RNA-protein crosslinker. AMT-NHS is composed of a psoralen derivative and an N-hydroxysuccinimide ester group which react with RNA bases and primary amines of protein, respectively. AMT-NHS can penetrate into living yeast cells and crosslink Cbf5 to H/ACA snoRNAs with high specificity. AMT-NHS induces different crosslinking patterns and targets both single- and double-stranded regions of RNA. AMT-NHS can be used for capturing diverse RNA-protein interactions in cells[1].

Product Specifications

CAS Number

[2925268-86-4]

UNSPSC

12352005

Target

Biochemical Assay Reagents

Type

Reference compound

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/amt-nhs.html

Purity

98.70

Solubility

DMSO : 25 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

O=C(ON1C(CCC1=O)=O)CCSCC2=C(C)OC(C(C)=C3O4)=C2C=C3C(C)=CC4=O

Molecular Formula

C22H21NO7S

Molecular Weight

443.47

References & Citations

[1]Han Y, et al. Development of an RNA-protein crosslinker to capture protein interactions with diverse RNA structures in cells. RNA. 2022 Mar;28 (3) :390-399.