Anti-α 2c Adrenergic Receptor Antibody

Mouse Monoclonal Antibody specific to α-2C Adrenergic Receptor

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

α-2 adrenergic receptor subtype C4, α-2C adrenoreceptor, α-2C adrenoceptor, α-2CAR

Gene Name

ADRA2C

NCBI Gene ID

UniProt

P18825

Cellular Locus

Cell membrane; Multi-pass membrane protein.

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to aa 442-462 (QDFRRSFFKHILFRRRRRGFQ), the cytoplasmic C-terminus, of human Alpha-2C adrenergic receptor (accession no. P18825).

Target Antigen

α-2C adrenergic receptor

Target

α 2c Adrenergic Receptor

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins.

Additionnal Information

Immunoblotting: use at 1ug/mL. Predicted molecular weight is ~50kDa or larger (possibly due to dimerization). <br><br>These are recommended concentrations. <br><br>Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human and mouse Alpha-2C adrenergic receptor.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

The alpha-2C adrenergic receptor (also known as ADRA2C) controls the release of neurotransmitters from central adrenergic neurons and from sympathetic nerves in the heart. There are three subtypes of the alpha-2 adrenergic receptor: alpha-2A, alpha-2B, and alpha-2C. The human alpha-2A subtype undergoes phosphorylation and short-term desensitization, whereas the human alpha-2C subtype is not phosphorylated and does not desensitize. The human alpha-2C subtype, however, does undergo arrestin-dependent internalization. In contrast to alpha-2A and alpha-2B adrenergic receptors from several species, the human alpha-2C receptor does not appear to down-regulate in response to agonist treatment in transfected cell culture systems.