Anti-Glutathione Reductase Antibody

Mouse Monoclonal Antibody specific to Glutathione Reductase

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

GR, GRase, EC 1.8.1.7

Gene Name

GSR

NCBI Gene ID

UniProt

P00390

Accession Number

NP_000628

Cellular Locus

[Isoform Mitochondrial]: Mitochondrion.; [Isoform Cytoplasmic]: Cytoplasm.

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human glutathione reductase purified from E. coli expressing the human glutathione reductase gene (aa 44-522).

Target Antigen

Glutathione reductase, mitochondrial

Target

Glutathione Reductase

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ELISA

Field of Research

Enzymes

Purification Method

PEG Purified

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Maintains high levels of reduced glutathione in the cytosol.

Additionnal Information

ELISA: Titration of glutathione reductase MAbs with glutathione reductase at 3ug/ml on the wells.ELISA Pairs: Both of these antibodies function as capture and detection antibodies at 1ug/ml. Immunoblotting: use at 0.5-1.0ug/ml. A band of ~57kDa is detected. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

These antibodies are stable for at least one (1) year at -20°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Specificity

These antibodies recognize human glutathione reductase. They do not cross-react with yeast glutathione reductase.

Formulation

PBS, pH 7.4

Buffer pH

pH 7.4

Target Background

Glutathione reductase, also known as glutathione-disulfide reductase, is an enzyme that in humans is encoded by the GSR gene. Glutathione reductase catalyzes the reduction of glutathione disulfide to the sulfhydryl form glutathione (GSH), which is a critical molecule in resisting oxidative stress and maintaining the reducing environment of the cell. The importance of the glutathione pathway and enzymes that affect it is gaining an increased level of attention. Although glutathione reductase has been an attractive target for many pharmaceuticals, there have been no successful glutathione reductase related therapeutic compounds created to date.