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Mouse anti Human IgD (class specific), conjugated with Biotin

Product Specifications

Background

To identify the presence of IgD in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgD, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

CAS Number

9007-83-4

Host

Mouse

Isotype

IgG1

Clone

NI 158

Conjugation

Biotin

Source

Highly purified polyclonal IgD isolated from Human serum.

Assay Principle

ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Form

Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7,2); Nopreservative added, as it may interfere with the antibody activity; Noforeign protein added

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Storage Conditions

The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C; Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day
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