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Chicken anti Bovine Serum Albumin (BSA)

Product Specifications

CAS Number

9007-83-4

Host

Chicken

Species Reactivity

Bovine

Conjugation

Affinity Purified

Type

Primary Antibodies

Source

Chickens were immunized with highly purified bovine serum albumin. After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction prepared. The antibodies were immunoaffinity purified from a solid phase immunoabsorbant.

Applications

WB, ELISA

Purification Method

Egg-yolk derived IgY Affinity Purified

Assay Principle

Recommended starting dilution for ELISA: 1:200-1:500 capture. 1:1,000-1:20,000 primary.

Concentration

See vial for Concentration

Form

Phosphate buffered saline‚ pH 7,2 with 0,075% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

References & Citations

1. Jun Isoe, Alberto A. Rasc on Jr., Susan Kunz, Roger L. Miesfeld. 2009. Molecular genetic analysis of midgut serine proteases in Aedes aegypti mosquitoes. Insect Biochemistry and Molecular Biology 39: 903-12 2. Alabaster, A., Isoe, J., Zhou G., Lee A., Murphy A., Day W.A. and R.L. Miesfeld. 2011. Deficiencies in acetyl-CoA carboxylase and fatty acid synthase 1 differentially affect eggshell formation and blood meal digestion in Aedes aegypti. Insect Biochemistry and Molecular Biology, 41:946-955. 3. Isoe J. and P.Y. Scaraffia. 2013. Urea Synthesis and Excretion in Aedes aegypti Mosquitoes Are Regulated by a Unique Cross-Talk Mechanism. PLoS ONE 8(6): e65393. doi:10.1371/journal.pone.0065393.

Storage Conditions

Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

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