Chicken anti Bovine Serum Albumin (BSA)
Product Specifications
CAS Number
9007-83-4
Host
Chicken
Species Reactivity
Bovine
Conjugation
Affinity Purified
Type
Primary Antibodies
Source
Chickens were immunized with highly purified bovine serum albumin. After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction prepared. The antibodies were immunoaffinity purified from a solid phase immunoabsorbant.
Applications
WB, ELISA
Purification Method
Egg-yolk derived IgY Affinity Purified
Assay Principle
Recommended starting dilution for ELISA: 1:200-1:500 capture. 1:1,000-1:20,000 primary.
Concentration
See vial for Concentration
Form
Phosphate buffered saline‚ pH 7,2 with 0,075% sodium azide
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
References & Citations
1. Jun Isoe, Alberto A. Rasc on Jr., Susan Kunz, Roger L. Miesfeld. 2009. Molecular genetic analysis of midgut serine proteases in Aedes aegypti mosquitoes. Insect Biochemistry and Molecular Biology 39: 903-12
2. Alabaster, A., Isoe, J., Zhou G., Lee A., Murphy A., Day W.A. and R.L. Miesfeld. 2011. Deficiencies in acetyl-CoA carboxylase and fatty acid synthase 1 differentially affect eggshell formation and blood meal digestion in Aedes aegypti. Insect Biochemistry and Molecular Biology, 41:946-955.
3. Isoe J. and P.Y. Scaraffia. 2013. Urea Synthesis and Excretion in Aedes aegypti Mosquitoes Are Regulated by a Unique Cross-Talk Mechanism. PLoS ONE 8(6): e65393. doi:10.1371/journal.pone.0065393.
Storage Conditions
Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
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