Human B7-H5 / Gi24 / VISTA Protein, Mouse IgG2a Fc Tag, low endotoxin

Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.

Product Specifications

Background

Specifications

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 45.0 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAH20568.1

Expression Region

Phe 33 - Ala 194

Host

HEK293

Target

B7-H5

Conjugation

Unconjugated

Tag

C-mFc (mIgG2a)

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated Human VSIG3, Fc,Avitag (Cat. No. VS3-H82F9) at 5 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human B7-H5 Protein, Mouse IgG2a Fc Tag (Cat. No. B75-H5258) with a linear range of 0.039-0.625 μg/mL (QC tested).