Anti-SNAT1 Neurotransmitter Transporter Antibody

Mouse Monoclonal Antibody specific to SNAT1 Neurotransmitter Transporter

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Amino acid transporter A1, rATA1, Glutamine transporter, N-system amino acid transporter 2, Solute carrier family 38 member 1, System A amino acid transporter 1, System A transporter 2, System N amino acid transporter 1

Gene Name

Slc38a1

NCBI Gene ID

UniProt

Q9JM15

Cellular Locus

Cell membrane

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 1-63 (cytoplasmic N-terminus) of rat SNAT1 (accession no. NP_620187.1).

Target Antigen

Sodium-coupled neutral amino acid transporter 1

Target

SNAT1 Neurotransmitter Transporter

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA. {PubMed:10660562, PubMed:11692272, PubMed:11756489, PubMed:12684517}.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of ~50kDa is detected. Immunohistochemistry: use at 1-5ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Rat neocortical neurons cultured under amino acid starvation conditions.

Storage Conditions

This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat SNAT1.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

The sodium-coupled neutral amino acid transporters (SNAT) include System A subtypes SNAT1, SNAT2, and SNAT4 and System N subtypes SNAT3 and SNAT5. These transporters are essential for uptake of nutrients, energy production, metabolism, detoxification, and the cycling of neurotransmitters. SNAT1 is responsible for transport of glutamine and may be involved in the generation of glutamate in the retina. SNAT1 is found in heart, brain, and placenta, and expression levels are high in certain neuronal populations in the central nervous system.