EcoPlexTM Mouse Th1/Th2/Th17 14-Plex Panel Kit

IL-2:P04351 TNF-α:P06804 IL-1α:P01582 IL-12p70:P43431&P43432 IFN-γ:P01580 IL-22:Q9JJY9 IL-13:P20109 IL-17A:Q62386 IL-4:P07750 IL-1β:P10749 IL-6:P08505 IL-5:P04401 IL-10:P18893 IL-21:Q9ES17

EcoPlexTM CBA kits utilize antibodies-conjugated beads which are different in size and mean fluorescent intensity of labeling dye to capture antigens in sample. After capture beads and sample incubation, biotin-conjugated detection antibodies are added to react with antigen-beads complexes, followed by a streptavidin-R-phycoerythrin (SA-PE) treatment. The fluorescent intensity of PE on each bead populations is quantified by flow cytometer with either a single 488nm laser or dual 488nm and 633/640nm lasers. The maximum emission of the bead classification dye is at 700nm. The concentrations of protein interested are determined by comparing the fluorescent intensities to those of standard curve, like sandwich ELISA data analysis.

IL-2<2pg/mL TNF-α<0.5pg/mL IL-1α<2pg/mL IL-12p70<1pg/mL IFN-γ<0.5pg/mL IL-22<5pg/mL IL-13<5pg/mL IL-17A<1.5pg/mL IL-4<0.5pg/mL IL-1β<2pg/mL IL-6<3pg/mL IL-5<5pg/mL IL-10<2pg/mL IL-21<5pg/mL

This kit is for detection of IFN-γ, IL-1α, IL-1β, IL-2 , IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-22 and TNF-α in mouse serum, plasma, biological fluid and cell culture supernatant.