Mammalian Lysis Buffer

<strong>Mammalian Lysis Buffer</strong>_x000D_ <table width="230">_x000D_ <tbody>_x000D_ <tr>_x000D_ <td width="143">Catalog number:</td>_x000D_ <td width="87">B2010099</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Lot number:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Expiration date:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Volume/Weight</td>_x000D_ <td>50 mL</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>pH</td>_x000D_ <td>7.5</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Supplied as</td>_x000D_ <td>Ready-to-Use</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Appearance</td>_x000D_ <td>Clear Solution</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Applications</td>_x000D_ <td>Mammalian Cell Lysis Buffer has been optimized for extraction of total soluble proteins from mammalian cultured cells.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Shelf-Life</td>_x000D_ <td>1 year from date of manufacture</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Storage:</td>_x000D_ <td>Keep at 2-8°C</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Keywords:</td>_x000D_ <td>Mammalian Cell Lysis Buffer, Cell Lysis Buffer</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Grade</td>_x000D_ <td>Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered on a 0.22 um.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>References</td>_x000D_ <td width="87">1: Desai N, Rambhia P, Gishto A. Human embryonic stem cell cultivation:_x000D_ historical perspective and evolution of xeno-free culture systems. Reprod Biol_x000D_ Endocrinol. 2015 Feb 22;13:9._x000D_ _x000D_ 2: Ruedinger F, Lavrentieva A, Blume C, Pepelanova I, Scheper T. Hydrogels for_x000D_ 3D mammalian cell culture: a starting guide for laboratory practice. Appl_x000D_ Microbiol Biotechnol. 2015 Jan;99(2):623-36._x000D_ _x000D_ 3: Vidmar J, Chingwaru C, Chingwaru W. Mammalian cell models to advance our_x000D_ understanding of wound healing: a review. J Surg Res. 2017 Apr;210:269-280._x000D_ _x000D_ 4: Jandt U, Platas Barradas O, Pörtner R, Zeng AP. Mammalian cell culture_x000D_ synchronization under physiological conditions and population dynamic_x000D_ simulation. Appl Microbiol Biotechnol. 2014 May;98(10):4311-9._x000D_ _x000D_ 5: Pappas D. Microfluidics and cancer analysis: cell separation, cell/tissue_x000D_ culture, cell mechanics, and integrated analysis systems. Analyst. 2016 Jan_x000D_ 21;141(2):525-35._x000D_ _x000D_ 6: Hong H, Park SJ, Han SJ, Lim J, Kim DS. Aquatic flower-inspired cell culture_x000D_ platform with simplified medium exchange process for facilitating cell-surface_x000D_ interaction studies. Biomed Microdevices. 2016 Feb;18(1):3._x000D_ _x000D_ 7: Neunstoecklin B, Villiger TK, Lucas E, Stettler M, Broly H, Morbidelli M,_x000D_ Soos M. Pilot-scale verification of maximum tolerable hydrodynamic stress for_x000D_ mammalian cell culture. Appl Microbiol Biotechnol. 2016 Apr;100(8):3489-98._x000D_ _x000D_ 8: Lim JM, Jo YM, Ahn JY. Laboratory control and basic culture protocols for_x000D_ stem cell self-renewal. Methods Mol Biol. 2015;1212:1-20._x000D_ _x000D_ 9: Barbulovic-Nad I, Au SH, Wheeler AR. A microfluidic platform for complete_x000D_ mammalian cell culture. Lab Chip. 2010 Jun 21;10(12):1536-42._x000D_ _x000D_ 10: Arden N, Betenbaugh MJ. Life and death in mammalian cell culture: strategies_x000D_ for apoptosis inhibition. Trends Biotechnol. 2004 Apr;22(4):174-80._x000D_ _x000D_ 11: Iles LR, Bartholomeusz GA. Three-Dimensional Spheroid Cell Culture Model for_x000D_ Target Identification Utilizing High-Throughput RNAi Screens. Methods Mol Biol._x000D_ 2016;1470:121-35._x000D_ _x000D_ 12: Huang SB, Chou D, Chang YH, Li KC, Chiu TK, Ventikos Y, Wu MH. Development_x000D_ of a pneumatically driven active cover lid for multi-well microplates for use in_x000D_ perfusion three-dimensional cell culture. Sci Rep. 2015 Dec 16;5:18352.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Related Products</td>_x000D_ <td><a href="https://moleculardepot.com/product-category/buffers/">Biochemical Buffers and Solutions</a></td>_x000D_ </tr>_x000D_ </tbody>_x000D_ </table>