Anti-Estrogen Receptor-a (Phospho-Ser167) Antibody

Rabbit Polyclonal Antibody specific to Estrogen Receptor-a (Phospho-Ser167)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Estrogen Receptor-a (Phospho-Ser167) Rabbit Polyclonal Antibody

Gene Name

Era

NCBI Gene ID

UniProt

G1E6Z3

Host

Rabbit

Reactivity

Human

Immunogen

Peptide sequence that includes phosphorylation site of Serine 167 (L-A-S(p)-T-N) derived from human estrogen receptor-a and conjugated to KLH.

Target Antigen

Estrogen receptor a

Target

Estrogen Receptor-a (Phospho-Ser167)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC, IF

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~66kDa is detected. Immunofluorescence: use at dilution of 1:100-1:200. Immunohistochemistry: use at dilution of 1:50-1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human estrogen receptor-alpha only when phosphorylated at serine 167.Accession no.: P03372, NP_000116.2

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

Estrogen receptor alpha (ERalpha), also known as NR3A1 (nuclear receptor subfamily 3, group A, member 1), is one of two main types of estrogen receptor, a nuclear receptor that is activated by estrogen. In humans, ERalpha is encoded by the gene ESR1 (Estrogen Receptor 1). Estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ESR1 mRNA transcripts, which differ primarily in their 5-prime untranslated regions. The translated receptors show less variability.