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P2RX4/P2X4 Antibody

P2RX4/P2X4 Antibody

Product Specifications

Product Name Alternative

P2RX4, ATP receptor, p2X purinoceptor 4, p2X4R, p2RX4, p2X receptor 4, p2X4, p2X4 purinoceptor, Purinergic receptor P2X4, Purinoceptor P2X4, Purinergic receptor, p2X receptor, subunit 4

Reactivity

Canine, Hamster, Human, Mouse, Rat

Immunogen

Peptide with sequence C-ETDSVVSSVTTKAK, from the internal region of the protein sequence according to NP_035156.2.

Target

P2RX4 / P2X4

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.5 mg/ml

Dilution

IF (10 μg/ml), IHC, IHC-P (5 μg/ml), Peptide-ELISA (1:16000), WB (0.3 - 1 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 60kD band observed in Human Kidney and Liver lysates (calculated MW of 43.5kD according to NP_002551.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Brown et al, J Biol Chem. 2004 Sep 17;279 (38) :39485-94. PMID: 15262999) . Recommended concentration: 1-3 ug/ml.

Tested Applications

ELISA, IF, IHC, IHC-P, WB

Host or Source

Goat

Preservative

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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