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MICU1/CBARA1 Antibody

MICU1/CBARA1 Antibody

Product Specifications

Product Name Alternative

MICU1, Ara CALC, CBARA1, CALC, EFHA3, Mitochondrial calcium uptake 1, Allergen Hom s 4, Atopy related autoantigen, Atopy-related autoantigen CALC

Reactivity

Bovine, Canine, Equine, Gallus, Goat, Hamster, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat, Xenopus, Zebrafish

Immunogen

Peptide with sequence C-EYENRIRAYSTPDK, from the internal region of the protein sequence according to NP_006068.2NP_001182447.1.

Target

MICU1 / CBARA1

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.5 mg/ml

Dilution

IHC, IHC-P (3.75 μg/ml), Peptide-ELISA (1:32000), WB (0.5 - 2 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 55kD band observed in lysates of cell line K562 (calculated MW of 54.6kD according to NP_006068.2) . Recommended concentration: 0.5-2 ug/ml. An additional band of unknown identity was also consistently observed at 37kD. This band was successfully blocked by incubation with the immunizing peptide.

Tested Applications

ELISA, IHC, IHC-P, WB

Host or Source

Goat

Preservative

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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