BRD4 Antibody
BRD4 Antibody
Product Specifications
Product Name Alternative
BRD4, Bromodomain containing 4, Chromosome-associated protein, MCAP, HUNK1, Protein HUNK1, Bromodomain-containing 4, HUNKI
Reactivity
Human
Immunogen
Synthetic peptide located between aa651-700 of human BRD4 (O60885-2, NP_055114) . Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Lizard (100%) .
Target
BRD4
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Immunoaffinity purified
Concentration
0.5 mg/ml
Dilution
IHC, IHC-P (10 μg/ml), WB (0.2 - 1 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml.
Tested Applications
IHC, IHC-P, WB
Host or Source
Rabbit
Preservative
PBS, 0.09% sodium azide, 2% sucrose
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