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ADGRA3/GPR125 Antibody

ADGRA3/GPR125 Antibody

Product Specifications

Product Name Alternative

ADGRA3, GPR125, TEM5L, G protein-coupled receptor 125, PGR21

Reactivity

Bovine, Equine, Hamster, Human, Mammal, Monkey, Mouse, Rabbit

Immunogen

Peptide with sequence CTNKSASSFKNS, from the internal region of the protein sequence according to NP_660333.2.

Target

ADGRA3 / GPR125

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.5 mg/ml

Dilution

IHC, IHC-P (3.75 μg/ml), Peptide-ELISA (1:8000), WB (0.5 - 1.5 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 140kD band observed in Human Ovary lysates (calculated MW of 146kD according to NP_660333.2) . Recommended concentration: 0.5-1.5 ug/ml. An additional band of unknown identity was also consistently observed at 33kD. This band was successfully blocked by incubation with the immunizing peptide.

Tested Applications

ELISA, IHC, IHC-P, WB

Host or Source

Goat

Preservative

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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