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Collagen IV Antibody

Collagen IV Antibody

Product Specifications

Reactivity

Bovine, Human, Porcine, Rat

Immunogen

Full length Type IV collagen isolated from human placenta

Target

Collagen IV

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Antiserum

Concentration

1 mg/ml

Dilution

ICC, IHC, IHC-Fr (1:25 - 1:100), IHC-P (1:75 - 1:100), WB (1:100 - 1:1000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Methanol fixation recommended for immunocytochemistry. For immunohistochemistry on frozen tissues (fixed in methanol, aceton or 1% paraformaldehyde) a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 is recommended. For immunohistochemistry on paraffin tissue the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature.

Tested Applications

ICC, IHC, IHC-Fr, IHC-P, WB

Host or Source

Rabbit

Preservative

Antiserum, 0.09% sodium azide

Isotype

IgG

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