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KEAP1 Antibody

KEAP1 Antibody

Product Specifications

Product Name Alternative

KEAP1, Cytosolic inhibitor of Nrf2, INrf2, KLHL19, Kelch-like protein 19, KIAA0132

Reactivity

Bovine, Canine, Equine, Human, Mammal, Monkey, Porcine

Immunogen

Peptide with sequence C-EVTPSQHGNRTFS, from the internal region (near N-Terminus) of the protein sequence according to NP_036421.2.

Target

KEAP1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.5 mg/ml

Dilution

IF (5 μg/ml), IHC, IHC-P (3.75 μg/ml), Peptide-ELISA (1:32000), WB (0.2 - 0.6 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 75kD band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 69.7kD according to Human NP_036421.2 and of 69.6kD according to Mouse NP_057888.1) . Recommended concentration: 0.2-0.6 ug/ml.

Tested Applications

ELISA, IF, IHC, IHC-P, WB

Host or Source

Goat

Preservative

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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