CXCL11 Antibody

CXCL11, Beta-R1, Chemokine h174, Chemokine ip-9, Chemokine itac, H174, I-TAC, IP9, ITAC, IP-9, SCYB11, Small inducible cytokine B11, B-R1, C-X-C motif chemokine 11, SCYB9B, Small-inducible cytokine B11

Resuspension: Reconstitute with sterile water. Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure) . A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hI-TAC (100 ng/ml), a concentration of 7-8 ug/ml of this antibody is required. ELISA: To detect hI-TAC by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human I-TAC as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hI-TAC. Western Blot: To detect hI-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-TAC is 1.5-3 ng/lane, under either reducing or non-reducing conditions.