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ROBO1 Antibody

ROBO1 Antibody

Product Specifications

Product Name Alternative

ROBO1, Deleted in U twenty twenty, H-Robo-1, Roundabout 1, SAX3, DUTT1, Roundabout homolog 1

Reactivity

Canine, Human, Mouse, Rat

Immunogen

This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an C-Terminal region near amino acids 1625-1650 of Human ROBO-1.

Target

ROBO1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Affinity purified

Concentration

1.1 mg/ml

Dilution

ELISA (1:30000 - 1:60000), IF, IHC, IHC-P (2.5 μg/ml), IP, WB (1:500 - 1:3000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 2.5 ug/ml.

Tested Applications

ELISA, IF, IHC, IHC-P, IP, WB

Host or Source

Rabbit

Preservative

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Isotype

IgG

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