BTC/Betacellulin Antibody

Resuspension: Reconstitute with sterile water. Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure) . A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human Betacellulin (0.1 ng/ml), a concentration of &le. 0.1 ug/ml of this antibody is required. ELISA: To detect hBetacellulin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Betacellulin as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBetacellulin. Western Blot: To detect hBetacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBetacellulin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.