DEFB1/BD-1 Antibody
DEFB1/BD-1 Antibody
Product Specifications
Product Name Alternative
DEFB1, BD1, Beta-defensin 1, BD-1, Beta Defensin 1, DEFB-1, DEFB101, Defensin, beta 1, Defensin beta, HBD-1, Beta-defensin-1, HBD1
Reactivity
Human
Immunogen
E.coli derived recombinant Human BD-1.
Target
DEFB1 / BD-1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Immunoaffinity purified
Dilution
ELISA (0.5 - 2 μg/ml), IHC, IHC-P (5 μg/ml), WB (0.1 - 0.2 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Resuspension: Reconstitute with sterile water to 0.1-1.0 mg/ml.. Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure) . A concentration of 5 ug/ml is recommended. ELISA: To detect hBD-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BD-1 as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBD-1. Western Blot: To detect hBD-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, IHC, IHC-P, WB
Host or Source
Rabbit
Preservative
Lyophilized from PBS, pH 7.2
Isotype
IgG
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