IL4 Antibody
IL4 Antibody
Product Specifications
Product Name Alternative
IL4, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, BCGF1, BSF-1, Binetrakin, BCGF-1, Interleukin 4, Interleukin-4, Pitrakinra, BSF1, IL-4
Reactivity
Human
Immunogen
E.coli derived recombinant Human IL-4.
Target
IL4
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Immunoaffinity purified
Dilution
ELISA (0.5 - 2 μg/ml), IHC, IHC-P (5 μg/ml), WB (0.1 - 0.2 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Resuspension: Reconstitute with sterile water. Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure) . A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-4 (1.50 ng/ml), a concentration of 03-05 ug/ml of this antibody is required. ELISA: To detect hIL-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-4 as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-4. Western Blot: To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, IHC, IHC-P, WB
Host or Source
Rabbit
Preservative
Lyophilized from PBS, pH 7.2
Isotype
IgG
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