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IL22 Antibody

IL22 Antibody

Product Specifications

Product Name Alternative

IL22, Cytokine Zcyto18, ILTIF, Interleukin 22, IL-22, IL-D110, TIFIL-23, Zcyto18, IL-TIF, Interleukin-22

Reactivity

Human

Immunogen

E.coli derived recombinant Human IL-22.

Target

IL22

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Immunoaffinity purified

Dilution

ELISA (0.5 - 2 μg/ml), IHC, IHC-P (5 μg/ml), WB (0.1 - 0.2 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Resuspension: Centrifuge the vial before opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex. . Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure) . A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-22 (50 ng/ml), a concentration of 1.25 ug/ml of this antibody is required. ELISA: To detect hIL-22 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-22 as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-22. Western Blot: To detect hIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-22 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Tested Applications

ELISA, IHC, IHC-P, WB

Host or Source

Rabbit

Preservative

Lyophilized from PBS, pH 7.2

Isotype

IgG

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