CCL20/MIP-3-Alpha Antibody
CCL20/MIP-3-Alpha Antibody
Product Specifications
Product Name Alternative
CCL20, Beta chemokine exodus-1, Beta-chemokine exodus-1, CKb4, Exodus-1, MIP-3a, MIP-3-alpha, LARC, ST38, SCYA20, C-C motif chemokine 20, CC chemokine LARC, Mip-3alpha, MIP3A, Small-inducible cytokine A20
Reactivity
Human
Immunogen
E.coli derived recombinant Human MIP-3alpha.
Target
CCL20 / MIP-3-Alpha
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein A purified
Dilution
ELISA (2 - 4 μg/ml), IHC, IHC-P (10 μg/ml), WB (0.2 - 0.4 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Resuspension: Centrifuge the vial before opening. Reconstitute in sterile water to 0.1 mg/ml.. Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure) . A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant Human MIP-3alpha when used with biotinylated antigen affinity purified anti-Human MIP-3alpha as the detection antibody at a concentration of approximately 1-2 ug/ml. Western Blot: To detect Human MIP-3alpha by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3alpha is 0.25-0.50 ng/lane, under non-reducing conditions.
Tested Applications
ELISA, IHC, IHC-P, WB
Host or Source
Mouse
Preservative
Lyophilized from PBS
Isotype
IgG1, k
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