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PTMA/Prothymosin Alpha Antibody

PTMA/Prothymosin Alpha Antibody

Product Specifications

Product Name Alternative

PTMA, Gene sequence 28, Prothymosin, alpha, TMSA, Prothymosin alpha, Prothymosin alpha protein

Reactivity

Bovine, Equine, Hamster, Human, Monkey

Immunogen

Peptide with sequence C-DVDTKKQKTDEDD, from the C-Terminus of protein sequence according to NP_001092755.1NP_002814.3.

Target

PTMA / Prothymosin Alpha

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.5 mg/ml

Dilution

IHC, IHC-P (3.75 μg/ml), Peptide-ELISA (1:1000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Peptide ELISA: antibody detection limit dilution 1:1000. Western blot: Preliminary experiments gave an approx. 38kDa band in lysates of cell line A431 after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 12.2kDa according to NP_001092755.1 and of 12.1kDa according to NP_002814.3. The 38kDa band was successfully blocked by incubation with the immunizing peptide.

Tested Applications

ELISA, IHC, IHC-P

Host or Source

Goat

Preservative

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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