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ANGPT1/Angiopoietin-1 Antibody

ANGPT1/Angiopoietin-1 Antibody

Product Specifications

Product Name Alternative

ANGPT1, Angiopoietin 1, Angiopoietin-1, AGPT, AGP1, ANG-1, ANG1, KIAA0003

Reactivity

Canine, Equine, Human, Monkey, Mouse, Rabbit

Immunogen

Peptide with sequence C-PDFSSQKLQH, from the internal region of the protein sequence according to NP_001137.2.

Target

ANGPT1 / Angiopoietin-1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cardiovascular Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.5 mg/ml

Dilution

IHC, IHC-P (2.5 μg/ml), Peptide-ELISA (1:32000), WB (1 - 2 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: In transfected HEK293 transiently expressing full-length Human ANGPT1 (myc and DYKDDDDK tagged), a band of approx. 48kD was observed. No bands were observed in mock-transfected HEK293 and the 48kD plus a 75kD band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2 ug/ml.

Tested Applications

ELISA, IHC, IHC-P, WB

Host or Source

Goat

Preservative

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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