NES/Nestin Antibody
NES/Nestin Antibody
Product Specifications
Product Name Alternative
NES, Nestin, Nbla00170
Reactivity
Human, Mouse
Immunogen
Anti-Nestin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1484-1500 of human Nestin protein.
Target
NES / Nestin
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Affinity purified
Concentration
1.23 mg/ml
Dilution
ELISA (1:20000 - 1:80000), IHC (5 μg/ml), IHC-P (5 μg/ml), WB (1:500 - 1:3000)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and a panel of mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.
Tested Applications
ELISA, IHC, IHC-P, WB
Host or Source
Rabbit
Preservative
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Isotype
IgG
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