RELA/NFKB p65 Antibody
RELA/NFKB p65 Antibody
Product Specifications
Product Name Alternative
RELA, NF-kappa-B p65delta3, NF-kappaB, NFKB3, p65, Transcription factor p65, NF kappaB, NFKB
Reactivity
Human, Mouse, Rat
Immunogen
NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) .
Target
RELA / NFKB p65
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Delipidated and defibrinated
Concentration
80 mg/ml
Dilution
ChrIP, ELISA (1:5000), GS, IHC, IHC-P (1:500), WB (1:2000 - 1:5000)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 1:500.
Tested Applications
ChIP, ELISA, Enzyme Assay, IHC, IHC-P, WB
Host or Source
Rabbit
Preservative
0.01% Sodium Azide
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