P16INK4a/CDKN2A Antibody
P16INK4a/CDKN2A Antibody
Product Specifications
Product Name Alternative
INK4A, Multiple tumor suppressor 1, MTS-1, MTS1, p16-INK4A, p16 INK4A, p16INK4A, CDKN2A, cyclin-dependent kinase inhibitor 2A
Reactivity
Human
Immunogen
Human p16 protein (missing the first 7 amino acids)
Target
P16INK4a / CDKN2A
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Delipidated and defibrinated
Concentration
85 mg/ml
Dilution
ELISA (1:5000 - 1:20000), IHC, IHC-P (1:500), IP (1:100), WB (1:500 - 1:5000)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 1:500.
Tested Applications
ELISA, IHC, IHC-P, IP, WB
Host or Source
Rabbit
Preservative
Antiserum, 0.01% Sodium Azide
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