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P16INK4a/CDKN2A Antibody

P16INK4a/CDKN2A Antibody

Product Specifications

Product Name Alternative

INK4A, Multiple tumor suppressor 1, MTS-1, MTS1, p16-INK4A, p16 INK4A, p16INK4A, CDKN2A, cyclin-dependent kinase inhibitor 2A

Reactivity

Human

Immunogen

Human p16 protein (missing the first 7 amino acids)

Target

P16INK4a / CDKN2A

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Delipidated and defibrinated

Concentration

85 mg/ml

Dilution

ELISA (1:5000 - 1:20000), IHC, IHC-P (1:500), IP (1:100), WB (1:500 - 1:5000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 1:500.

Tested Applications

ELISA, IHC, IHC-P, IP, WB

Host or Source

Rabbit

Preservative

Antiserum, 0.01% Sodium Azide

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