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SLC12A2/NKCC1 Antibody

SLC12A2/NKCC1 Antibody

Product Specifications

Product Name Alternative

SLC12A2, Basolateral Na-K-Cl symporter, BSC2, MBSC2, NKCC1, Sy-ns, BSC

Reactivity

Bovine, Canine, Equine, Gallus, Human, Porcine

Immunogen

Peptide with sequence C-NALQHSIRLSGVEDH, from the internal region of the protein sequence according to NP_001037.1NP_001243390.1.

Target

SLC12A2 / NKCC1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.5 mg/ml

Dilution

IF (10 μg/ml), IHC, IHC-P (3.75 μg/ml), Peptide-ELISA (1:64000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave an approx 75kDa band in Human, Mouse and Rat Skeletal Muscle, Heart and Brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 131kDa according to NP_001037.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide.

Tested Applications

ELISA, IF, IHC, IHC-P

Host or Source

Goat

Preservative

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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