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SYT1/Synaptotagmin Antibody

SYT1/Synaptotagmin Antibody

Product Specifications

Product Name Alternative

SYT1, SYT, SVP65, Synaptotagmin, Synaptotagmin 1, SytI, Synaptotagmin I, Synaptotagmin-1

Reactivity

Bovine, Canine, Equine, Gallus, Hamster, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat, Zebrafish

Immunogen

Peptide with sequence C-RDLQSAEKEEQEK, from the internal region of the protein sequence according to NP_005630.1.

Target

SYT1 / Synaptotagmin

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.5 mg/ml

Dilution

IHC, IHC-P (3.75 μg/ml), Peptide-ELISA (1:2000), WB (0.3 - 1 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Approx. 65kDa band observed in Human Brain (hippocampus) and Mouse and Rat Brain lysates (calculated MW of 47.6kDa according to NP_005630.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Matthew et al, J Cell Biol. 1981 Oct;91 (1) :257-69.; PMID: 7298720) . Recommended concentration: 0.3-1 ug/ml.

Tested Applications

ELISA, IHC, IHC-P, WB

Host or Source

Goat

Preservative

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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