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DNASE2/DNase II Antibody

DNASE2/DNase II Antibody

Product Specifications

Product Name Alternative

DNASE2, Acid DNase, DNase II, DNase II alpha, DNASE2A, Deoxyribonuclease II alpha, Deoxyribonuclease-2-alpha, DNase II, lysosomal, R31240_2, DNL, DNL2, Lysosomal DNase II

Reactivity

Human

Immunogen

A 14 AA peptide near the C-Terminus of human DNase II. The immunogen is located within the last 50 AA of DNase II.

Target

DNASE2 / DNase II

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Peptide Column Affinity Chromatography

Concentration

1 mg/ml

Dilution

ELISA, IF, IHC, IHC-P (5 μg/ml), WB (1:500 - 1:1000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: DNase II antibody can be used for detection of DNase II expression by Western blot at 0.5 ug/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Aprox: 40 kDa

Tested Applications

ELISA, IF, IHC, IHC-P, WB

Host or Source

Rabbit

Preservative

PBS containing 0.02% Sodium Azide

Isotype

IgG

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