ABCB1/MDR1/P Glycoprotein Antibody

ABCB1, ABC20, Abcb1b, CLCS, Colchicin sensitivity, CD243, IBD13, gp170, MDR1, Multidrug resistance protein 1, P glycoprotein, P-glycoprotein 1, P-GP, CD243 antigen, PGY1

Further information: JSB-1 has potential value for diagnostic detection of MDR cells in human tumor samples. Working dilutions. Flow cytometry: at least 1:10. Western blotting: start optimizing working dilution a 1:10. Immunocytochemistry: acetone fixed cell preparation at least 1:20. Immunohistochemistry: acetone-fixed frozen sections at least 1:20, formalin-fixed paraffin embedded tissues 1:20. Instructions for use Optimal staining results are obtained with routine 2-step ABC or APAAP methods using acetone-fixed cytocentrifuge preparations or cryostat sections. In case the B-5 fixative is used (see below) also paraffin embedded tissue can be stained with the antibody. In Flow cytometry fixate cells in 10% (v/v) Lysing solution followed by primary antibody and antiMouse-FITC. In developing a Western blot staining pattern use an anti-Mouse-HRP.