TGF-Beta Antibody

Product Specifications

Reactivity

Human

Immunogen

TGF-b from human platelets.

Target

TGF-Beta

Clonality

Monoclonal

Clone

TB21

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G purified

Concentration

1 mg/ml

Dilution

ELISA, Func, IHC, IHC-Fr, IHC-P (10 μg/ml), Neut, WB

References & Citations

Jia-Shuo Kan et al. SIX2-Mediated Microglial M2 Polarization and Exosomal miR-3470b Delivery Protect Dopaminergic Neurons in Parkinson's Disease CNS Neurosci Ther ., (2026)

Pubmed id

41700504

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Suitable for use in Western Blot, ELISA, Neutralization and Immunohistochemistry. Neutralization: Neutralizes TGF-beta activity in vitro in an inhibition assay of CCL/64 cell growth and the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of microinjection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo. Immunohistochemistry (formalin-fixed, paraffin-embedded, frozen) : 1:1000. Has been used to demonstrate TGF-beta in ovine ovarian tissue and human breast carcinoma. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue. Western Blot: 5-20 ng/ml will allow visualization of 100 ng/lane of TGF-beta. Recognizes the dimeric (25kD) and monomeric (12.5kD) forms of TGF-beta under both non-reducing and reducing conditions respectively.