HMG-1 (Acetyl Lys3) rabbit pAb

Function: Binds preferentially single-stranded DNA and unwinds double stranded DNA. similarity: Belongs to the HMGB family. similarity: Contains 2 HMG box DNA-binding domains.

Product Specifications

Background

Function:Binds preferentially single-stranded DNA and unwinds double stranded DNA., similarity:Belongs to the HMGB family., similarity:Contains 2 HMG box DNA-binding domains.

UniProt

P09429

Swiss Prot

P09429

Reactivity

Human; Mouse; Rat

Immunogen

Synthesized peptide derived from human HMG-1 (Acetyl Lys3)

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

WB 1:1000-2000 ELISA 1:5000-20000

Molecular Weight

about 30kd

Storage Conditions

-20°C/1 year

Observed Molecular Weight

About 30kd

Fragment

IgG

Subcellular Location

Nucleus . Chromosome . Cytoplasm . Secreted . Cell membrane ; Peripheral membrane protein ; Extracellular side . Endosome . Endoplasmic reticulum-Golgi intermediate compartment . In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus (PubMed:12231511, PubMed:17114460) . Translocates from the nucleus to the cytoplasm upon autophagy stimulation (PubMed:20819940) . Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes (By similarity) . Passively released to the extracellular milieu from necrotic cells by diffusion, involving the fully reduced HGMB1 which subsequently gets oxidized (PubMed:19811284) . Also released from apoptotic cells (PubMed:16855214, PubMed:18631454) . Active secretion from a variety of immune a