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FZD2/Frizzled 2 Antibody

FZD2/Frizzled 2 Antibody

Product Specifications

Product Name Alternative

FZD2, Dfrizzled2, Fz2, Frizzled family receptor 2, Frizzled-2, HFz2, Frizzled homolog 2, Fz-2, FzE2, Mfz10, Rfz2, Frizzled 2

Reactivity

Bovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rat

Immunogen

Peptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1.

Target

FZD2 / Frizzled 2

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.5 mg/ml

Dilution

IF (10 μg/ml), IHC, IHC-P (2.5 μg/ml), Peptide-ELISA (1:64000), WB (0.03 - 0.1 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx. 23kD band in Human Colon, Heart and Ovary lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.6kD according to NP_001457.1. The 23kD band was successfully blocked by incubation with the immunizing peptide.

Tested Applications

ELISA, IF, IHC, IHC-P, WB

Host or Source

Goat

Preservative

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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