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FMR1/FMRP Antibody

FMR1/FMRP Antibody

Product Specifications

Product Name Alternative

FMR1, FMRP, POF, Protein FMR-1, Premature ovarian failure 1, Fragile X mental retardation 1, FRAXA, POF1

Reactivity

Hamster, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat

Immunogen

Peptide with sequence C-RTGKDRNQKKEKPD, from the internal region (near the C-Terminus) of the protein sequence according to NP_002015.1.

Target

FMR1 / FMRP

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.5 mg/ml

Dilution

IHC, IHC-P (2.5 μg/ml), Peptide-ELISA (1:128000), WB (0.1 - 0.3 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 80kD band observed in lysates of cell line KELLY (calculated MW of 71.2kD according to NP_002015.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sittler et al, Hum Mol Genet. 1996 Jan;5 (1) :95-102; PMID: 8789445) . Recommended concentration: 0.1-0.3 ug/ml.

Tested Applications

ELISA, IHC, IHC-P, WB

Host or Source

Goat

Preservative

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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