Complement C4d Antibody
Complement C4d Antibody
Product Specifications
Reactivity
Human
Immunogen
Peptide derived from internal sequence of human C4.
Target
Complement C4d
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Purified
Concentration
1.4 mg/ml
Dilution
IHC, IHC-Fr (1:100 - 1:200), IHC-P (1:25 - 1:100)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for This was determined to be 1:50.
Tested Applications
IHC, IHC-Fr, IHC-P
Host or Source
Rabbit
Preservative
20 mM Tris-HCl, pH 8.0, 20 mg/ml rAlbumin, 0.05% Sodium Azide
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