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KRT14/CK14/Cytokeratin 14 Antibody

KRT14/CK14/Cytokeratin 14 Antibody

Product Specifications

Product Name Alternative

KRT14, CK-14, EBS3, EBS4, K14, Keratin-14, CK14, Cytokeratin 14, Cytokeratin-14, Keratin 14, NFJ

Reactivity

Human

Immunogen

Peptide derived from C-Terminus of human cytokeratin 14.

Target

KRT14 / CK14 / Cytokeratin 14

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Purified

Dilution

IHC, IHC-Fr (1:100 - 1:200), IHC-P (1:25 - 1:100)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for This was determined to be 1:50.

Tested Applications

IHC, IHC-Fr, IHC-P

Host or Source

Rabbit

Preservative

20 mM Tris-HCl, pH 8.0, 20 mg/ml rAlbumin, 0.05% Sodium Azide

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