NT5E/eNT/CD73 Antibody
NT5E/eNT/CD73 Antibody
Product Specifications
Product Name Alternative
NT5E, 5-nucleotidase, ecto (CD73), 5'-nucleotidase, 5 nucleotidase (CD73), 5-NT, 5-nucleotidase, 5'-nucleotidase, ecto (CD73), CD73, CD73 antigen, E5NT, Ecto-5-nucleotidase, ENT, EN, NT, Purine 5-Prime-Nucleotidase, NT5, NTE
Reactivity
Human
Immunogen
Purified recombinant fragment of NT5E expressed in E. coli.
Target
NT5E / eNT / CD73
Clonality
Monoclonal
Clone
1D7
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Ascites
Dilution
ELISA (1:10000), IHC, IHC-P (1:200 - 1:400)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for This was determined to be 1:200.
Tested Applications
ELISA, IHC, IHC-P
Host or Source
Mouse
Preservative
Ascitic fluid, 0.03% sodium azide
Isotype
IgG1
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