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PEA3/ETV4 Antibody

PEA3/ETV4 Antibody

Product Specifications

Product Name Alternative

ETV4, ETS translocation variant 4, E1A-F, E1AF, Ets variant 4, PEA3, Protein PEA3, PEAS3

Reactivity

Human

Immunogen

Purified recombinant fragment of human ETV4 (aa50-109) expressed in E. coli.

Target

PEA3 / ETV4

Clonality

Monoclonal

Clone

1A2G3

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Ascites

Concentration

1 mg/ml

Dilution

ELISA (1:10000), IHC, IHC-P (1:200), WB (1:100 - 1:500)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for This was determined to be 1:200.

Tested Applications

ELISA, IHC, IHC-P, WB

Host or Source

Mouse

Preservative

Ascitic fluid, 0.03% sodium azide

Isotype

IgG1

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