WNT5A Antibody
WNT5A Antibody
Product Specifications
Product Name Alternative
WNT5A, HWNT5A, Protein Wnt-5a, WNT-5A protein
Reactivity
Human
Immunogen
Purified recombinant fragment of WNT5A expressed in E. Coli.\
Target
WNT5A
Clonality
Monoclonal
Clone
3D10
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Ascites
Dilution
ELISA (1:10000), IHC, IHC-P (1:200 - 1:400), WB (1:100 - 1:500)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for This was determined to be 1:200
Tested Applications
ELISA, IHC, IHC-P, WB
Host or Source
Mouse
Preservative
Ascitic fluid, 0.03% sodium azide
Isotype
IgG1
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