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WNT5A Antibody

WNT5A Antibody

Product Specifications

Product Name Alternative

WNT5A, HWNT5A, Protein Wnt-5a, WNT-5A protein

Reactivity

Human

Immunogen

Purified recombinant fragment of WNT5A expressed in E. Coli.\

Target

WNT5A

Clonality

Monoclonal

Clone

6F2

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Ascites

Dilution

ELISA (1:10000), IF (1:200 - 1:1000), IHC, IHC-P (1:200 - 1:400), WB (1:100 - 1:500)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for This was determined to be 1:200.

Tested Applications

ELISA, IF, IHC, IHC-P, WB

Host or Source

Mouse

Preservative

Ascitic fluid, 0.03% sodium azide

Isotype

IgG1

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